Annotated Bibliography of Articles on Health Communication

Health Communication Annotated Bibliography Brezina, Corona. (2010). Organ Donation: Risks, Rewards, and Research. Rosen Pub: New York, NY. An important role of the healthcare professional will have to do with the acceptance and request for organ donations. When a patient passes away, their loved ones will be asked if the organs of the deceased can be harvested. The healthcare professional must be able to make this request with enough tact that the bereaved will not be offended. The Hippocratic Oath that doctors and nurses must take in order to become medical professionals forces them to pledge they will First Do No Harm. Cutting into healthy bodies is mutilation and the opposite of this pledge (Brezina 10). So, is the cutting into a human body in order to retrieve an organ for an unhealthy one a similar violation of this credo? In order to find new medical treatments, there is often an experimental stage wherein first lower life forms and then more advanced beings are experimented with. The thesis being that the ends will justify the means. The experimentation will yield a greater good and the suffering of one will benefit the many. This is the same working theory behind organ transplants. Although one person will suffer if the organ is retrieved from a living body, the benefits to the person needing the organ will outweigh the unhappiness. A life here has more value than a single organ and it is the harvesting of a single organ that will save an entire life. PeopleShow MoreRelatedA Annotated Bibliography On The Field Of Nursing1600 Words   |  7 PagesAn annotated bibliography is used to assess other written works of information related to the topic you are discussing. 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The Basic Principles of Topics for Synthesis Essay You Can Benefit From Beginning Right Away

The Basic Principles of Topics for Synthesis Essay You Can Benefit From Beginning Right Away Your own essay should be constructed around that text and your principal argument. Be certain that you leave a potent conclusion when writing your essay. The title ought to be a reflection of those. Remember that the absolute most important part of a synthesis essay is to demonstrate the reader an extensive comprehension of the subject. It may require a significant amount of details and evidence. Before you write, it's important to define the term synthesis. The term synthesis is understood to be a blend of elements to form a connected whole. Getting the Best Topics for Synthesis Essay Becoming able to compose a comprehensive, thorough and meaningful essay, with various sources in hand might end up being problematic for some. The principal ideas gathered from the close reading ought to be utilized to justify the usage of a particular source. Once you work through your sources, make a decision as to what position you're likely to take. What you will need is a topic that has many sources that may support more than 1 position. The New Angle On Topics for Synthesis Essay Just Released Parenting topics have a broad array of formulations, based on a subject they're related to. Synthesis topics should not be something which's general expertise, like whether vegetables are good for you. You can look for other fantastic topic ideas on the world wide web. The topic ought to be handled in a detailed and objective way. Selecting a topic is the initial step of your synthesis essay writing, so you've got to put an outstanding effort in choosing one. It is preferable to choose such a topic which you are already well-conversant of. Whatever They Told You About Topics for Synthesis Essay Is Dead Wrong...And Here's Why However you're feeling about the respective topics you have to present your case utilizing evidence from credible sources and create the case your perspective on the problem is correct or at least noteworthy. As you experience an argument, do not neglect to supply your readers with the counter-argument. Now you have your argument down in words, you want to work out how you need to organize and support that argument. Then show the way your argument is stronger. Most Noticeable Topics for Synthesis Essay Once more, the body of the essay should start ou t with information regarding the paragraph's topic. Somehow, this issue of your upcoming essay is dependent on what you would like to do by your paper and the sources you rely on. A synthesis essay is a sort of essay that supposes a distinctive viewpoint on a chosen idea and is contingent on the mixture of many sources, usually three or more. Therefore, synthesis essays involve assembling several elements of information together on a particular topic and presented in a way about the thesis statement. Synthesis essays are typical in high school and college classes hence they might not be a totally new phenomenon to some people. Thus, a synthesis is the sort of essay which offers you as the student several resources to utilize in building a strong claim. The dissertation services make certain that your thesis paper won't be pushed to the wayside. Why Almost Everything You've Learned About Topics for Synthesis Essay Is Wrong You should explain the central facets of a specific phenomenon logically and straightforwardly in order for your audience does not have any trouble understanding it. Why the election method isn't fair. Lousy synthesis topics can arrive in a number of forms. The topic you select for your paper needs to be relevant to the area and society. Keep in mind it's important to choose the synthesis essay type. A potent conclusion is necessary for your synthesis essay. You ought to choose synthesis topics that aren't only intriguing but also reasonable to research. A complete topic for a synthesis paper is one which encourages you to have a stance on a debatable iss ue. Critical thinking and analysis is needed to make sure high excellent synthesis essay content. If you are not able to do proper proofreading, you can get an expert at Essay Agents look at your synthesis essay for a reasonable price. Topics for Synthesis Essay and Topics for Synthesis Essay - The Perfect Combination Space exploration isn't an exception. Doing research on this issue you really like will allow you to find out more about things that fascinate you and you are certain to take pleasure in the process. Guided by means of a set of instructions, you should support your ideas in favor of the specific topic. Focus on a single part of climate change or current environmental problems that you're wondering about. It isn't simple to concentrate on a single topic whenever the options are by no means limited. Limit your topic options just a few but attempt to concentrate on what's really interesting to you.

Aspect of International Business Enterprise - Myassignmenthelp.Com

Question: Discuss about the Aspect of International Business Enterprise. Answer: Introduction Due to globalisation and digitalisation, it is easier for organisations to expand their operations over worldwide which increase the number of multinational companies. International corporations have to comply with domestic and international regulatory frameworks that are established by the government. A firm can gain competitive advantage and increase its brand image by effectively comply with its regulatory framework. This report will analyse the regulatory framework of BHP Billiton and evaluate various laws that are necessary to comply by the corporation while operating its business in Australian market. BHP Billiton is a multinational mining, petroleum, and metals company which was founded in 1885. The firm performs a wide variety of oil and gas, mining and processing producing operations in more than 25 countries (BHP, 2017). Further, the report will focus on analysing various treaties, agreements, and conventions that influence the business of BHP Billiton. BHP Billiton Broken Hill Proprietary Company Limited merged with Billiton PLC in 2001, and the new corporation was established called BHP Billiton; it is an Anglo-Australian international metal, petroleum, and mining firm. It is a dual listed company, and it is listed on both London Stock Exchange and Australian Securities Exchange (BHP, 2017). The corporation focuses on establishing an utmost level of governance fostering a culture which incorporates corporate integrity and ethical standards into the business structure of the firm. The company values sustainable development of its stakeholders and give utmost importance its safety and health of its employees; the enterprise has prioritised its responsibility towards protecting the environment, and it contributes towards the development of local communities. According to Forbes (2017), BHP Billiton is one of the worlds top multinational performer companies with a market capitalisation of US$98.3 billion. Industry of BHP Billiton The corporation operates in metal and mining industry, and it is one of the worlds largest depositors of uranium, gold, coal, nickel, iron, and copper. The operations of the company are situated in more than 25 countries, and it is one of the leading resource firms worldwide. In 2016, the enterprise had revenue of AU$30.9 billion from its operations worldwide (Owler, 2017). Number of Staff in Australia and Globally BHP Billiton is a world-leading resource firm with more than 65,263 employees, and contractors situate throughout the world. In 2017, the corporation has more than 26,146 employees in Australia which include employees from all subsidiaries; the CEO of the enterprise is Andrew Mackenzie (IBIS World, 2017). Location of Global Headquarters BHP Billitons global headquarters is located at 171 Collins Street, Melbourne, Australia (BHP, 2013). The main operations of the corporations are situated in Australia, United States, United Kingdom, Brazil, and Canada. Regulatory Framework of BHP Billiton BHP Billiton is a key player in the Australian market, and various provisions influence its operations. The current Australia regulations require corporations to maintain transparency in their operations and focus on sustainable development. Following are various provisions and regulations that influence the operations of BHP Billiton. The Australian Jobs Act As per the provisions of Australian Jobs Act, 2013, the developing and manufacturing firms of major projects and products with a capital expenditure of more than AU$500 million are compulsorily required by the government to execute and draft an Australian Industry Participation Plan (AIPP). The motive of this plan is to ensure that corporations are providing full, reasonable and fair opportunities to other enterprises while operating in the industry of Australia relating to supply goods and services (Competition Policy Review, 2014). The intention of government increases the regulatory and administrative burden on producing corporations such as BHP Billiton. The firm has to claim that it is ethically complying with various regulations relating to penetration of competition. BHP Billiton has undermined productivity due to compliance with regulations, and it further increased the expenses of firm and project delays. The corporation might face detrimental impact because of disclosing se nsitive information to third party suppliers and competitors. Petroleum Resource Rent Tax and Mineral Resource Rent Tax The government of Australia has imposed 30 percent tax on mineral resources which resulted in imposing 22.5 percent additional tax on profits of corporations which deals in coal and iron ore operations. BHP Billitons import and export operations are also affected by Petroleum resource rent tax (The Australian, 2015). DomGas Policy The Australian government has introduced Western Australias Domestic Gas Policy, 2006 (DomGas) with the intention of lowering the prices of domestic gas than compared to free-market equilibrium rates; due to the regulations of policy, the gas supply investment in Australia has reduced considerably. BHP Billiton is Australias largest gas and oil producers, and its operations are directly affected by the policy (Domgas, 2010). The policy introduces new economic burden for corporations such as BHP Billiton, which will eventually decrease their investment in gas supply and production. Due to this policy, the completion will reduce in the industry which will negatively influence Australias economy. Australian Shipping Regulations The Coastal Trading (Revitalising Australian Shipping) Act, 2012 has introduced new infrastructure for issuing license whereby it is significantly difficult and expensive for non-Australian vessels to provide intrastate and interstate shipping services. The new regulations have reduced the competition for BHP Billiton in the industry (Parliament of Australia, 2012). The shipping cost for outside countrys vessels has increased substantially due to this act which resulted in reducing the competition for Australian products that are transferred through this form of transportation. Through, the act has a negative impact on the profitability of BHP Billiton as well because it has surged the coastal shipping costs. Australian Competition Law The present competition law of Australia is significantly complex that focuses on statutory form. The law has negatively influenced the operations of BHP Billiton which affects its expenses and consistency among international regulations (BHP, 2017). Impact of Agreement, Treaties or Conventions on BHP Billiton Agreement on Iron Ore Production Joint Venture between BHP Billiton and Rio Tinto On 5th June 2005, BHP Billiton entered into an agreement with Rio Tinto for production joint venture. The agreement focuses the iron ore business of both the corporations and their assets (BHP, 2009). The agreement provides guidelines for various current and future assets and liabilities which are relating to iron ore business of both the firms. Both the enterprises will own similar roles and responsibilities relating to their iron ore production operations. The agreement assists in developing substantial cooperation between both the corporations while operating their iron ore business in Western Australia. The main objective of the agreement is to produce low-cost iron ore. As per the calculations of BHP Billiton and Rio Tinto, the development and production of their joint venture will result in generating more than 10 billion dollars for the corporations in the future. Royalty Amendment Agreement between BHP Billiton and the Government of Western Australia This agreement between the government of Western Australia and BHP Billiton cover various business operations of the enterprise relating to a royalty payment on iron ore shipment. The Western Australian Mining Regulations will specify the new rate of royalty for the iron ore consignment. BHP Billiton and the government of Western Australia have made amendments to various other documents, such as infrastructure for Rio Tintos permit sharing (IOL, 2010). After its amendment, this agreement will assist BHP Billiton in increasing the efficiency of its operations. The complexity of regulations will be reduced after this agreement which is beneficial for the enterprise since it increases its productivity and performance. The operating capabilities of the firm will be improved with the use of rail and infrastructure; the corporation will be able to produce more goods in the Australian market with the help of rail and infrastructure. Agreement to Undertake a Strategic Assessment on Iron Ore between BHP Billiton and Australian Government On 18th September 2012, BHP Billiton has entered into an agreement with Australian government for development of iron ore mines. The agreement focus on strategically assessing the planned evaluation of the influence of iron mines development in the future and analyse how it is associated with the national environmental significance. The agreement was constructed as per the provisions of the Environment Protection and Biodiversity Conservation Act 1999. The agreement is based on section 146(1) which provides provisions that are related to the policies and plans of the corporation and their implementation (Environment, 2017). In order to increase its effort in the protection of environmental resources, the enterprise has designed and set up the activities in iron ore operations to increase their efficiency. The agreement will assist BHP Billiton in strategically identifying and measuring various possible impacts of its on-going programs related to protection and management of practices . The information collected from the strategic measuring will assist the corporation in developing new plans that will assist in reducing their negative impact on the environment. These policies will assist the enterprise in maintaining their integrity and reputation in the industry and provide them various opportunities to enhance their support for developing its business. Strategic improvement in operation will increase the future business opportunities for the enterprise and assist them in fulfilling their corporate responsibilities (BHP, 2017). Therefore, the agreement assists BHP Billiton in developing quality products by improving their existing procedures. The corporation can easily provide high-quality products and offer better services to its customers with the help of this agreement which increases the profitability of the firm. Agreement with Banjima People BHP Billiton has entered into an agreement with Banjima people in Western Australia. The all-inclusive agreement provides the guidelines for business operations of the enterprises iron ore plant which is situated in Pilbara, and it covers the area of more than 8263 square kilometers. According to BHP Billiton, the agreement will assist in increasing the uniqueness and transparency in engaging with local indigenous communities. Due to this agreement, the Banjima people will get good opportunities to improve their future with the help of various projects and programs of the corporation (Shepherd, 2015). The programs assist in overall development of the indigenous people and improve various social, health and economic issue. The mining process will be done in areas which are selected by BHP Billiton which result in increasing the productivity of iron ore. Due to this agreement, the products of BHP Billiton will be easily available in the market at considerably low cost. Agreement between BHP Billiton and PT Alam Tri Abadi (Adaro) for Selling Interest in IndoMet Coal In, 2016, BHP Billiton contracted with its equity partner PT Alam Tri Abadi (Adaro) to sell 75 percent of its interest in IndoMet Coal (Hobbs, 2016). Although IndoMet Coal could support a large-scale development, BHP Billiton has various other growth options that are more attractive for its future investment. IndoMet Coal comprises seven leading coal contracts of work situated in Central and Eastern Kalimantan, Indonesia. The agreement was initiated to increase companys focus on the crucial area of business. The agreement will assist in bringing more future investment for the corporation that will influence the productivity and performance of the business. After this agreement, the enterprise is focusing on other future investment that will assist them in increasing their market share and sustain their future development. Conclusion Conclusively, multinational corporations deal with various regulatory frameworks which influence their business operations. BHP Billiton is one of the leading companies in diversified metal and mining industry. The firms operations are affected by the regulatory framework which is provided by the government of Australia. Effective compliance with regulatory framework ensures that corporations are complying with necessary legal requirements, and they are performing their duties ethically. The regulatory framework of BHP Billiton in Australia includes the Australian Jobs Act, the Coastal Trading (Revitalising Australian Shipping) Act, Western Australias Domestic Gas (DomGas) Policy, Australian Competition Law and many others. BHP Billiton has entered into various agreements which influence their business such as an agreement with the government of Western Australia, PT Alam Tri Abadi, Banjima People, Rio Tinto and many others. BHP Billiton should effectively comply with its regulatory framework since it results in increasing brand image and sustainable future development. References BHP. (2009). BHP Billiton and Rio Tinto sign binding agreements on Iron Ore Production Joint Venture. Retrieved from https://www.bhp.com/media-and-insights/news-releases/2009/12/bhp-billiton-and-rio-tinto-sign-binding-agreements-on-iron-ore-production-joint-venture BHP. (2013). BHP Billiton Opens New Global Head Office. Retrieved from https://www.bhp.com/media-and-insights/news-releases/2013/11/bhp-billiton-opens-new-global-head-office BHP. (2017). About Us. Retrieved from https://www.bhp.com/our-approach/our-company/about-us BHP. (2017). BHP Billiton Iron Ore Pilbara Strategic Assessment. Retrieved from https://www.bhp.com/-/media/bhp/regulatory-information-media/iron-ore/western-australia-iron-ore/0000/western-australia-iron-ore-pilbara-strategic-assessment-commonwealth/bhp-billiton-pilbara-strategic-assessment-program-final-4-may-20171.pdf BHP. (2017). Competition law compliance. Retrieved from https://www.bhp.com/our-approach/operating-with-integrity/competition-law-compliance BHP. (2017). Minerals Australia. Retrieved from https://www.bhp.com/our-businesses/minerals-australia Competition Policy Review. (2014). BHP Billiton submission to the Competition Policy Review Panel. Retrieved from https://competitionpolicyreview.gov.au/files/2014/06/BHP_Billiton.pdf Domgas. (2010). Western Australias Domestic Gas Security. Retrieved from https://www.domgas.com.au/pdf/Other_reports/DomGas_Report_2010.pdf Environment. (2017). Agreement to undertake a strategic assessment of the impacts of a Plan for mining iron ore in the Pilbara region, Western Australia. Retrieved from https://www.environment.gov.au/system/files/pages/c59ed6a7-e955-4df9-a8da-156ddd76e0eb/files/bhpbio-strategic-assessment-agreement.pdf Forbes. (2017). Top Multinational Performers. Retrieved from https://www.forbes.com/companies/bhp-billiton/ Hobbs, H. (2016). BHP selling its interest in IndoMet Coal. Retrieved from https://www.worldcoal.com/coal/07062016/bhp-selling-its-interest-in-indomet-coal-913/ IBIS World. (2017). BHP Billiton Limited - Premium Company Report Australia. Retrieved from https://www.ibisworld.com.au/australian-company-research-reports/mining/bhp-billiton-limited-company.html IOL. (2010). BHP in deal with Australian government. Retrieved from https://www.iol.co.za/business-report/international/bhp-in-deal-with-australian-government-804125 Owler. (2017). BHP Billiton. Retrieved from https://www.owler.com/iaApp/12105325/bhp-billiton-company-profile Parliament of Australia. (2012). Coastal Trading (Revitalising Australian Shipping) Bill 2012 [and] Coastal Trading (Revitalising Australian Shipping) (Consequential Amendments and Transitional Provisions) Bill 2012. Retrieved from https://www.aph.gov.au/Parliamentary_Business/Bills_Legislation/bd/bd1112a/12bd151 Shepherd, B. (2015). Banjima people celebrate after Pilbara native title deal signed in Kings Park ceremony. Retrieved from https://www.abc.net.au/news/2015-11-04/bhp-billiton-and-banjima-people-sign-pilbara-native-title-deal/6913522 The Australian. (2015). Minerals Resources Rent Tax raised more than expected. Retrieved from https://myaccount.news.com.au/sites/theaustralian/subscribe.html?sourceCode=TAWEB_WRE170_a_GGLmode=premiumdest=https://www.theaustralian.com.au/business/mining-energy/minerals-resource-rent-tax-raises-more-than-expected/news-story/50b67cf186009a3efcd6e38e876d11camemtype=anonymous

Traditional VS. Digital Painting Essay Example For Students

Traditional VS. Digital Painting Essay Many have seen the outcomes to be very similar to each Other. Digital painting ND painting by hand might have the same visual looks, however, these two methods are very different when creating the art piece. Digital painting is one of the newest methods that is being taught to new artist. This type of method is where an artist can create art on the computer using a graphics tablet. When an artist decides to do a digital painting; they do not need to spend as much money. All that they need to purchase is their laptop or computer, a graphics drawing tablet such as a Waco tablet, a digital painting aerogram, and the canvas the artist will print on. At first, that will all seem like a great amount of money, which contradict what was stated above, but, that is all that is mostly needed to buy once. The program will have brushes, strokes, colors, and other materials included, These supplies have amazing effects that are able to imitate the real materials, such as how watercolors bleeds on paper. We will write a custom essay on Traditional VS. Digital Painting specifically for you for only $16.38 $13.9/page Order now So, all the artist really needs to purchase is the printing materials each time they make new art Another beneficial trait to digital painting, is that there is no mess included because in the program, there is a tab called the color palette which acts like real palette where an artist mixes their colors. If a mistake occurs, they are able to undo or fix it very easily because it has these digital channels that are called layers. In the layers, pieces of the artwork can be worked on and if there is an error or mistake, theres no need to delete the Whole art piece. Digital painting makes it easy to save the artists work as different formats incase they want to do different effects on Other programs such as Photos. Being able to save or change the format is very beneficial to an artist trying to grow or show their portfolio. They are also able to put the artwork aside to work on it later or if they are busy. This can be a pro or con in digital painting, because if an artist needs more inspiration they can put it off to the side, but, if they put it off for too long then they might lose motivation to continue. As for the most traditional method, painting by hand is the most common and studied. This kind of painting can be very expensive. Compared to digital painting t is way more money. Depending on what kind of artist is painting, they might want very high quality paint and materials or be living on their art and paint very often. And when an artist paints very often, they will most likely need to keep buying materials. Most of the common materials needed are the brushes, paints, palettes, canvas, and even materials that are optional to the artist. Painting by hand is very messy, regardless if there is a tarp all over the floor, paint will get on it or the artist. This kind of method takes a lot of mixing to find he right color or to make many colors. If there is a mistake made, it can be difficult to be undone and it might just have to be paint on by the correct color. In worst case scenarios, the artwork might have to be started all over. TO save as a portfolio it is suggested to take a professional picture and a to save it digitally. Saving a painting for later is possible but difficult to do. The artist might have to buy a special palette that requires a storage that wont dry the paint. This method does seem difficult to handle but in the end it is worth it. .ub898497b6efacc1760de0202309877e9 , .ub898497b6efacc1760de0202309877e9 .postImageUrl , .ub898497b6efacc1760de0202309877e9 .centered-text-area { min-height: 80px; position: relative; } .ub898497b6efacc1760de0202309877e9 , .ub898497b6efacc1760de0202309877e9:hover , .ub898497b6efacc1760de0202309877e9:visited , .ub898497b6efacc1760de0202309877e9:active { border:0!important; } .ub898497b6efacc1760de0202309877e9 .clearfix:after { content: ""; display: table; clear: both; } .ub898497b6efacc1760de0202309877e9 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .ub898497b6efacc1760de0202309877e9:active , .ub898497b6efacc1760de0202309877e9:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .ub898497b6efacc1760de0202309877e9 .centered-text-area { width: 100%; position: relative ; } .ub898497b6efacc1760de0202309877e9 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .ub898497b6efacc1760de0202309877e9 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .ub898497b6efacc1760de0202309877e9 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .ub898497b6efacc1760de0202309877e9:hover .ctaButton { background-color: #34495E!important; } .ub898497b6efacc1760de0202309877e9 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .ub898497b6efacc1760de0202309877e9 .ub898497b6efacc1760de0202309877e9-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .ub898497b6efacc1760de0202309877e9:after { content: ""; display: block; clear: both; } READ: Andy Warhol Essay FameSome Of the most beautiful paintings in history were created this way. Compared to painting by hand, digital painting is way more beneficial to an artist. Digital painting is clean and painting by hand is very messy. Both can be expensive, but, an artist wont purchase more when painting on the computer. And as for painting by hand, as traditional as it is, the quality can be very expensive and artist are more likely to buy more of what they need over and over again. Even if both have their cons and pros; the artist may choose any preference and art will be art.

Global Population essays

Global Population essays One of the biggest problems with the world today is our rapidly growing global population. With the current population being at over 6 billion, every second five people are born and two people die which means the population grows by 3 people per second. I predict that if we do not do something about this problem soon, the Earth is going to reach its maximum capacity and we will slowly run out of resources, which could cause wide-spread famine and eventually lead to the population dropping down to a normal level again, or perhaps there may be a natural disaster such as a worldwide earthquake that would leave only a select 1000 people living. We need to act now to prevent a disaster such as this happening. I believe that if we teach sex education properly in all of our schools, and the people of the world are able to accuire birth control easily when they need it we can stop this expeditiously growing problem. Our survival depends on population control and also better management of our precious natural resources. Because we are very limited in the amount, natural resources need to be managed accordingly. We should plan a new way to manage the planets natural resources. As our population grows, most of our problems will only worsen. These problems include air and water pollution, not enough fresh water, too many overcrowded schools, quality of life may become worse, taxes increase, crimes increase, people with out jobs or homes, soil pollution. The chance of having one natural disaster take down all of the population but a select few is extremely slim, but possible. Earth has a unique way of working, and if there are too many people on the planet, mother nature may take control. We need to find other methods of controlling the population so that we do not have to suffer through death or the death of loved ones. We can stop this problem, but it wont just happen, people have to do s...

Blackwater Draw - 12,000 Years of Hunting in New Mexico

Blackwater Draw - 12,000 Years of Hunting in New Mexico Blackwater Draw is an important archaeological site associated with the Clovis period, people who hunted mammoths and other large mammals in the North American continent between 12,500–12,900 calendar years ago (cal BP). When Blackwater Draw was first inhabited, a small spring-fed lake or marsh near what is now Portales, New Mexico was populated with extinct forms of elephant, wolf, bison, and horse, and the people who hunted them. Generations of many of the earliest occupants of the New World lived at Blackwater Draw, creating a layer cake of human settlement debris including Clovis (radiocarbon dated between 11,600–11,000 [RCYBP]), Folsom (10,800–10,000 years BP), Portales (9,800–8,000 RCYBP), and Archaic (7,000–5,000 RCYBP) period occupations. History of Blackwater Draw Excavations Evidence of the earliest occupation at what was to be known as the Blackwater Draw site was sent to the Smithsonian Institution in 1929, but full-scale excavation didnt happen until 1932 after the New Mexico roads department began quarrying in the neighborhood. Edgar B. Howard of the University of Pennsylvania Museum conducted the first excavations there between 1932–33, but he was hardly the last. Since then, excavators have included many of the best archaeologists in the New World. John L. Cotter, E. H. Sellards and Glen Evans, A.E. Dittert and Fred Wendorf, Arthur Jelinek, James Hester, and Jerry Harbour, Vance Haynes, William King, Jack Cunningham, and George Agogino all worked at Blackwater Draw, sometimes ahead of the sporadic gravel mining operations, sometimes not. Finally, in 1978, the site was bought by Eastern New Mexico University, who operate a small onsite facility and the Blackwater Draw museum, and to this day conduct archaeological investigations. Visiting Blackwater Draw Visiting the site is an experience not to be missed. In the intervening millennia since the prehistoric occupations of the site, the climate has dried out, and the remnants of the site now lie 15 feet and more below the modern surface. You enter the site from the east and wander down along a self-guided path into the depths of the former quarry operations. A large windowed shed protects the past and current excavations; and a smaller shed protects a Clovis-period hand-dug well, one of the earliest water control systems in the New World; and one of at least 20 total wells on-site, mostly dated to the American Archaic. The Blackwater Draw Museum website at Eastern New Mexico University has one of the best public programs describing any archaeological site. Go see their Blackwater Draw website for more information and pictures of one of the most important Paleoindian archaeological sites in the Americas. Selected Sources Andrews, Brian N., Jason M.  Labelle, and John D. Seebach. Spatial Variability in the Folsom Archaeological Record: A Multi-Scalar Approach. American Antiquity 73.3 (2008): 464–90. Print.Boldurian, Anthony T. Clovis Type-Site, Blackwater Draw, New Mexico: A History, 1929–2009. North American Archaeologist 29.1 (2008): 65–89. Print.Buchanan, Briggs. An Analysis of Folsom Projectile Point Resharpening Using Quantitative Comparisons of Form and Allometry. Journal of Archaeological Science 33.2 (2006): 185–99. Print.Grayson, Donald K., and David J. Meltzer. Revisiting Paleoindian Exploitation of Extinct North American Mammals. Journal of Archaeological Science 56 (2015): 177–93. Print.Haynes, C. Vance and James M. Warnica. Geology, Archaeology, and Climate Change in Blackwater Draw, New Mexico: F. Earl Green and the Geoarchaeology of the Clovis Type Site. Eastern New Mexico Contributions in Anthropology 15, 2012Seebach, John D. Stratigraphy and Bonebe d Taphonomy at Blackwater Draw Locality No. 1 During the Middle Holocene (Altithermal). Plains Anthropologist 47.183 (2002): 339–58. Print.

Differiating Between Market Structures Essay Example | Topics and Well Written Essays - 1000 words

Differiating Between Market Structures - Essay Example The first part of the paper will identify the market structure of the high technology industry; outline the market share of Samsung Corporation, explain why I decided upon this marketing structure and how different the marketing structure is from other alternatives. Part two of the paper will identify four competitive strategies that may be used by Samsung to maximize its profits effectively within the high tech industry’s competitive environment. More so the paper will evaluate the efficiency of these strategies in the market structure. Market structure of the high technology industry High technology industries are prone to the same market forces just like any other industry, but there exist few forces that are stronger in the high tech industry. Research claims that these forces affected the market structure of high technology industry in the 1890s and are still affecting the same industry to date. The high technology industry falls under an imperfectly competitive market st ructure, which means that products and services are highly differentiated (Varian, 2001). There are vast factors that arise from effective price discrimination that affects the high technology industry. These factors include personalization of products and pricing, versioning, bundling, switching costs, lock-in and economics of scale. Differentiation of products and services - The information technology allows flexible observation and analysis of consumer behavior, which in turn brings in various kinds of marketing strategies that seemed impossible on a large scale. For instance, a seller can offer prices and goods that are differentiated through individualized behavior and characteristics. Ideally, the high technology industry allows the market of one phenomenon where customers can personalize their prices (Varian, 2001). The high technology industry customers can personalize their adverts on the front pages online and can still buy a personally configured computer. According to th e theory of monopoly first degree price discrimination in most industries charge the highest prices they can to each customer, but the technology industry’s case is different because online sellers face competition from each other as well as from online sellers; hence, further stiffening the competition. Versioning - Versioning is a second-degree price discrimination that usually falls under product quality, which means that sellers design product line that appeals to different market segmentation. For instance, the latest trend of DVD version sells at a higher price and has a lot of content, an aspect that studios uses to discriminate prices between collectors and viewers, as well as between buyers and renters. Bundling - Bundling is the process of selling two or more distinct goods together for a single price. In the technology industry, these goods are â€Å"small† information goods like the individual items of web content that are sold in extremely large bundles. B undling affects the economy by reducing the dispersion of willingness to pay as well as increase entry barriers, but it enhances firm profits and overall efficiency (Varian, 2001). For instance, instead of a soft w3are company selling a word processor and a spreadsheet separately at different prices, the company could combine the two products and sell them at a higher price. Switching cost - Unlike other industries, switching costs in the high technolo

The need for divorce reform Essay Example | Topics and Well Written Essays - 2250 words

The need for divorce reform - Essay Example In 1966, the Law Commission undertook a study and noted that 40% of illegitimate births occurred in stable families, a factor that mooted reform in the divorce laws. There is a need to evaluate social evidence in making the estimation about the need for reform and the kinds of reform that will be appropriate. Proposals for reform in the divorce process were mooted by Government4 in a report that examined the viability of mediation as an option in handling divorce costs in view of rising costs that were accruing to contestants in a divorce. As a result of the need for reform that was identified in this report, the Family Law Act of 1996 was passed by Parliament in 1996, whereby divorce is granted on the basis of an irretrievable breakdown of a marriage. While this law introduces many useful amendments, the problem is that some of the provisions are still not operational and may not be brought into effect. Some of the major grounds that permit divorce are (a) adultery (b) unreasonable behavior (c) separation (d) two years separation with consent (e) five years separation without consent. The major causes of divorce are adultery and unreasonable behavior, reflecting the changing social mores that have created single parent families, step parents, same sex parents and extended family units. In view of the issues that rise in a divorce settlement, including high costs and the need to reduce acrimony in the divorce for the welfare of the children that may be involved in the divorce process, arbitration may emerge as a viable process.

Critical Review of Danger Boy Essay Example for Free

Critical Review of Danger Boy Essay Mark Williams’ â€Å"Danger Boy: Ancient fire† is set in 2019. It is a science fiction and time travelling novel. The text explores the effects of building a time machine. In Mark Williams’ novel, he examines the negative impact of futurist technology. Although the concept of time travelling may seem appealing at first glance, there is an ultimately destructive consequence that comes with it, like losing a love one. The novel opens with an argument between Eli’s father, Sandusky, and Mr Howe, a representative from a government department. Mr Howe persuades Sandusky to invent a machine that can slow down time in a force field. Sandusky’s wife, a scientist, assists Sandusky to invent the time travelling machine but she is sucked into the machine and travels back in time. Therefore, Sandusky is urged to produce another time machine that can slow down time with a more stable core. Upon starting up the machine, a piece of old paper emerges. Eli reaches for the newspaper and gets sucked into the year of Alexandria (415 C. E). Eli and his companion, a dinosaur and an Alexandrian, seeks to reunite Sandusky’s wife, returning to the present day life and save the world from the fatal disease that has slipped through time. The novel is structured by different narrative perspectives, in which different characters narrate the story from their personal point of view and experience. This type of structure of narrative point of views offers the reader a different perspective of the world from three characters: Eli, Clyne and Thea. The writer’s intention here may be to demonstrate the reality is never black or white, but can be interpreted and understood in different ways in different individuals. However, the one limitation to this type of structure is that the reader may experience confusion in attempting to understand the flow of the story, in which disputes the coherency of events. There are no obvious clues to indicate the change of the protagonist. The only visible clue available is the chapter title and the narrator’s name. Hence, this confuses us in terms of identifying the protagonist. As the reader continues to read, they will encounter an acronym many times before it gives its meaning. There are instances in the text when certain words such as â€Å"WHOMPEs† suddenly appear, which disrupts the flow of the story. Reading this novel for the first time will confuse the reader. As the reader progresses through the novel, Sandusky explains to Eli what a â€Å"WHOMPERs† is. It is a â€Å"Wide Orbiting Massless ParticlE Reversers. † The writers intention suggest that the scientific invention is an abstract idea which is foreign to humanity. The author uses a stream of consciousness to enable the reader to engage with the thought process of the character. When Sandusky answered Eli’s question about time, he replied ‘you go into the Fifth Dimension†¦hard to tell the difference between time and space, or when and where. Or even who and what. † This encourages the reader to think and be in the mind of the narrator. This is a really good idea for advanced readers who want to think beyond the novel. Williams’ got inspired to write the novel by his son. He got the idea when his son was running down the hallway yelling out â€Å"I’m a Danger Boy! † The novel’s setting is based on where he lives, which is LA. There could be a connection between the novel’s setting and where he lives. One connection is that LA is a place for relaxation and tourism. The main character travels to LA with his father to relax a bit after the tragic incident. One of the major themes in â€Å"Danger Boy† is time traveling. Time traveling is frequent in the novel. It happens when ever Eli puts his â€Å"WHOMPER† charged baseball cap on. Another theme them is history. History is shown in this novel from many perspectives. It is seen by going back in time to Alexandria (415 C. E). The main motif in the novel is a baseball cap. It is a motif because it’s a device that allows Eli to time travel. Without the baseball cap, Eli would just be like any other ordinary kid. This novel can be compared with Audrey Niffenegger’s â€Å"The Time Traveller’s Wife†. The two novels involve the same themes; history and time travelling. In â€Å"Danger Boy† Eli becomes a time traveller after he touches a time machine. He travels back in time to Alexandria. He can control when he wants to time travel, but can’t control on where it’ll take him. In â€Å"The Time Traveller’s Wife† Henry is born with a genetic disorder. This genetic disorder allowed him to time travel. There was one thing that he can’t control. It is where he lands from time travelling and when he time travels. Ultimately, Williams has created an adventurous and exciting novel. The book was exciting because it produced tension into whether the protagonists find Margret or not. The writer produced a text that was a bit difficult to read and establishment of the main character. The author shaped the words in the sentences really strangely which sometimes doesn’t make sense or it is not suitable, so the reader needs to re-read it to understand it. This novel should be raising questions about time travel, especially in the future.

Causing Generation X :: essays research papers

Teenagers wasting 2.3 million dollars worth of gas a year driving around the same block. Kids at the mall for the sole purpose of shop-lifting. Underage consumption parties. Stealing lawn ornaments. These are just some of the effects of Generation X growing up.   Ã‚  Ã‚  Ã‚  Ã‚  The main cause of this destruction of youth is the crumbling of families. No more “Wait until your father gets home from work and ask him'; but “I wonder if we’re going to see a child support check before Christmas.'; With the divorce rate skyrocketing, us kids are feeling more like burdens than blessings. All the problems divorce causes can rip apart a child, and leave him/her craving attention, whether it is negative or positive. To make it worse, while the only parent they live with is working, the two kids turn into a huge group of people decide to make prank phone calls and try beer for the first time. Addictions form and on and on. Pretty soon the kids acting up in class, (more attention, the perfect kind, peer,) getting smashed on the weekends and waking up in someone else’s puke.   Ã‚  Ã‚  Ã‚  Ã‚  Another big reason is boredom. This where my syndrome stems from. There is honestly NOTHING to do in Tuscarwaras County. If you have money in the winter you can bowl, watch a High School basketball game, go to the YMCA, see a movie, go shopping, drive around, experiment with make-up and different types of clothes, get on the Internet, and watch TV. With no money in the winter, we can watch TV, get on the Internet, vandalize, steal , try to bum cigarettes and other such things. The reason the police log is so long every morning is not because kids are heathens, it’s because we are bored. If there was a dance club, a clean place to play pool, an indoor swimming pool , etc., that was free, I guarantee you the problems would clear up. I huge issue that stands out is the whole bike/skateboard thing. Dover/Phila has a huge problem with us riding in town and in allies. I’ve been yelled at hundreds of times “This isn’t a bike park! Get the He*l away form here!'; Well, if they’d build us a place instead of whining about it for months and getting our hopes up then saying no. In order to keep us out of trouble, we need to be occupied with good things It’s not that we don’t want to be.

How Business Strategy and Hr Strategy Are or Should Be Linked Together

Institute of Tourism and Hotel Management Anna Morozova Essay How business strategy and HR strategy are or should be linked together? Moscow, 2011 Departments are the entities organizations form to organize people, reporting relationships, and work in a way that best supports the accomplishment of the organization's goals. Departments are usually organized by functions such as human resources, marketing, administration, and sales. The forward thinking human resource department is devoted to providing effective policies, procedures, and people-friendly  guidelines and support  within companies.Additionally, the human resource function serves to make sure that the company mission,  vision,  values  or  guiding principles, the company metrics, and the factors that keep the company guided toward success are optimized. Of executives surveyed, 20% currently use the HR department as active and innovative business solution partners. 20% believe that the HR department should remai n as administrative overhead and only perform transactional work. But, 60% of the  executives are starting to expect the HR department to partner with others departments to improve the company’s core competencies and competitive advantages.Competitive pressure in a fast changing business world – pressures for sales, talent, and profits. Most CEO’s are held accountable for three general but powerful results: Increasing revenue, generating cash, and reducing costs. In order to focus on these three accountabilities, executives are discarding paradigms that no longer work as companies seek to stay in and grow their business. Many CEOs and CFOs are more interested in the payoff and are asking appropriate questions: What’s in it for the company? Where is the improvement in the revenue stream? How does this get us new customers and retain our current customers.Where is the proof of corporate performance enhancement metrics? Once they get solid answers to these questions from competent HR leaders, the CEOs are quick to change their thinking. To answer the payoff questions, recognize that a continual company-wide value chain analysis is critical to the success of any organization. Over the past decade, CEOs began demanding that their Human Resources departments deliver flawless functional work and become a knowledgeable partner with all other disciplines to advance the business plan of the company. Individual professional silos are breaking down.Disciplines such as finance, sales, marketing, operations, and HR no longer exist as stand-alone entities. They are inter-dependent with one another. Weakness of any one of the links inhibits other links from maximizing their efficiency and productivity. These three emerging concepts in the practice of HR bear examination: * What value does the HR department brings to the organization. Many HR teams lack a vision that includes their value to the organization. Do the HR department’s activitie s directly help the company achieve its broad business objectives?Are the HR team’s arguments for or against a business strategy credible to the other department heads at the decision making table? How are the HR department strategies that benefit the employees, the shareholders, the customers, and all other stakeholders in the organization, selected and implemented? * What value does the HR department generate for the customer – the end user of the company’s product or service? Sales and quality are no longer restricted to the sales and quality assurance teams. The HR department doesn’t just hire a salesperson based upon a manager’s request.The end result of HR’s recruiting and hiring efforts is that the customer who interacts with the new sales person receives continuing world class service from the company. HR shares the quality of the new hire with the other departmental silos to insure that the company is, or becomes, the vendor of choi ce for that customer. * The final of the three emerging concepts for the Human Resources Department is: What core business competencies must HR leaders possess in order to be credible strategic partners with the rest of the executive team?Each company and each industry can generate its own list of core business skills their teams must have that go beyond their individual specialties. This issue has become so critical that in graduate and undergraduate level business programs, new editions of Organizational Development textbooks are including chapters on financial calculations and ratios, corporate social responsibility, globalization, and major workforce diversity challenges, among others.The biggest barrier to profitability is ignorance – ignorance by many people about how the company makes money and how it achieves its objectives, and how all of the departmental silos are interdependent on each other. The myth that only finance people need to know about finance or that mark eting people are the only people who need to know about marketing is fast disappearing. In today’s business environment, profitable organizations require highly skilled employees who can solve complex problems using multi-disciplinary teams.Here are three examples how can HR be linked to profitability metrics: * A well known global company formed a group of HR professionals who developed processes and training programs in sales, customer service, workouts, project management, process improvement and leadership development that focused on critical performance issues for their internal and external customers. By partnering with operations, sales, and customer service they served as a catalyst to forge alliances, partnerships and agreements.Many of their efforts resulted in improved relationships that translated into â€Å"Preferred Provider Status†, which increased sales and lowered costs. All of their costs were liquidated by charging a fee for the service while creatin g net revenue. After two years, this HR group generated sales of $4 million and a profit margin in excess of 30% which was returned to the division budget at the end of each fiscal year. * Secondly, an HR team, partnering with the Audit staff, discovered that the accounts receivable turnover had moved from a preferred 30 days to 45 days during the past two years.They decided to let the chief credit officer go. The HR staff established criteria to identify candidates with the ability to reduce the ratio from 45 days back to 30 days. The HR staff recommended one candidate for hire. Within six months, the company’s DSO (Days Sales Outstanding) ratio was reduced to 35 days. * In a third case, while designing and negotiating a new health care and 401(k) plan, the HR leadership partnered with the sales and marketing team to determine if the cost of the program would erode the company’s market share and competitive pricing strategy.The resulting benefit program design achieve d its cost/benefit objectives without jeopardizing the company’s market share and pricing metrics. How do HR leaders and CEOs make the Human Resources Department to a Profitability Factor? Here are suggestions based on that the more employees become knowledgeably involved in the business, the better they will be able to become a more productive asset. * Develop a leadership development program that includes hands on training in all of the functional disciplines.For example, in the production department, identify the barriers that prevent managers from achieving efficiencies and savings; * Insist that Human Resources staff receive financial training so they understand the impact of cash flow, receivables, billing cycles, and so forth. If it is a public company, teach them how to read and understand company’s annual report. Reading the proxy statement is always informative – even if the information contained in it is reluctantly revealed, and occasionally masked w ith arcane accounting jargon; * Have HR staff participate in sales strategies, customer visits, and technology reviews.Encourage them to learn quality methods, process improvements techniques, terms and conditions, and contract negotiations with suppliers and customers. Engage them as process consultants (have them trained if necessary) so they can assist with growth initiatives; * Most importantly, hold all employees accountable for achieving the â€Å"critical numbers† established for your company. A superb HR department becomes irrelevant if the company is sliding into bankruptcy. The HR department's powerful value focuses on its contributions toward reversing the slide.It is important to Include HR employees as full business partners. They will rise to the occasion and surprise you by building your bottom line and becoming a profit center contributor as well as maintaining their traditional responsibilities – and they will be better at both. The intense and brutall y competitive business environment of our global and digital world needs the help of everyone in the company. Russian small enterprises do not practice the establishment of HR Departments in view of unprofitability of such a business organization.As the result, the majority of small enterprises do not develop any HR strategy. Thus I would like to present the unique HR strategy of Apple Inc. and how it is linked to company’s business strategy. Most firms strive to have a productive workforce. One of the best ways to measure workforce productivity is revenue per employee. Apple produces what can only be considered extraordinary revenue per employee; $2 million. A second measure of workforce productivity is profit per employee: nearly $478,000 for Apple (unbelievable considering it has a retail workforce).During 25 years Apple has been following the philosophy called â€Å"lean – management† which explains the prime drivers for Apple’s extraordinary employee productivity. For years, the leadership of Apple has followed the philosophy that having less is more, meaning that by purposely understaffing and operating with reduced funding, you can make the team more productive and innovative. Innovation at most firms is expensive because you must pay for a lot of trial and error.The lean approach, however, can improve innovation because with everything being tried, there simply isn’t enough time or money for major misses and re-do’s. â€Å"Unrealistic deadlines† at Apple mean that you have to get project problems solved early on, because there isn’t time to redo things over and over. Being lean forces the team to be more cohesive. Even providing a lean schedule forces everyone to be productive because they know there is no room for slippage. At Apple, the lean approach means that even with its huge cash resources, every employee must adopt the mentality of leanness.If you understand the lean concept and its advant ages, you shouldn’t be surprised that numerous innovations have been developed in â€Å"garages,† the ultimate lean environment. I have chosen an article â€Å"Human resource practices to attract and retain talents† by Hiltrop, 1999, because, in my opinion, it is very actual theme as businesses look for global growth, chronic skills gaps combined with a mismatch between demand and supply of talent means that getting (and keeping) the right people in the right places at the right time has never been more challenging.HR leaders need to mobilize talent to help businesses grow. This article explores one of the biggest issues and challenges now faced by large organizations: how to attract and retain a critical group of talented people. Getting talent management right means you can worry less about your talent problems and more about your business opportunities. It is very important to use a fact based approach to help identify the specific elements of talent manageme nt which drive the most value in your business and industry.It is needed to create a Talent Management Framework and Diagnostic to develop and implement strategies that deliver the right improvements – those that give you the best return on investment. So I can conclude that to win the war for talent, companies should figure out who they are aiming for, and then make sure the recruitment process and practices are tailored to the specific needs and expectations of the target group. I have chosen an article â€Å"Science and practice of HRM in small firms† by Mayson and Barret, 2006 because human capital (i. e. the knowledge, skills, and abilities of employees) is one of the primary factors a business can rely on to differentiate their products or services and build a competitive advantage; however, few studies directly guide managers of small and growing firms through the people management issues that they will face through the lifecycle of their business. The recogniti on that human resource issues are important to small and growing firms is not new. For instance, in 1987 (Hess) was presented data that suggested that small business owners rank human resource related issues as the second most important management activity after general management.Further, was suggested that the majority of CEO's believe that human resource practices have a substantial impact on firm performance. Additionally, in 2008 were presented the results suggesting that sound hiring practices and training programs are considered important by small business owners who have 10 or more employees. A small firms' ability to attract, motivate and retain employees by offering competitive salaries and appropriate rewards is linked to firm performance and growth.Whilst the evidence does show that there is some form of HRM in small firms, it also confirms that the practice is characterized by informality. I personally think, that this is a problem, because informal HRM practices do not necessarily recognize the value of employees. Despite the recognition of the importance of HRM to small, growing and entrepreneurial firms, there is very little research in the area, there is even less research that explores the strategic nature of HRM in small firms.It is important to note, that properly developing strategic selection, training, and compensation programs takes time and financial resources. However, these short-term costs are almost always balanced by long term gain because the quality and caliber of employees (or human capital) within the firm improves. The improved caliber of employees and enhanced effort almost always has a positive financial impact for the organization.

Evaluate the Organization’s Involvement and Responsibility to the Community Essay

Evelyn Hamilton, Aaron Pobleh and Regina Taylor CJA/474: Managing Criminal Justice Personnel November 26, 2012 Instructor: Pam McIver The term motivation and empowerment is a universal concept that is hoped to work towards the good of any organization. Motivation and empowerment does not come from rewards to employees but instead, recognition, responsibility and advancement. Leaders who are effective in motivating and empowering their staff have acquired a great achievement, which can increase efficiency, and self development of skills and abilities. When employers are concerned about the welfare and needs of their staff, this provides trust among staff. The purpose of this paper is to compare motivation and empowerment, define the components of empowerment, analyze the implications of empowerment and delegation in a criminal justice organization and analyze the role of trust in personnel issues. Compare motivation and empowerment Motivation and empowerment are two words that are very popular in every workplace. Generally when one mention motivation, everyone would think it is what employer gives their employees, unfortunately, motivation in the workplace goes way far beyond that. Motivating your employees gives them authority to do more work for you and do it better. According to businessdictionary. com, motivation is both internal and external factors which desire and energize people to become committed to a job, role, or subject, or to strive to reach a goal. While empowerment is management practices which allow the sharing of information, power, and rewards with employees so that they can take initiative, and make decision to solve problems, and improve themselves as well as enhance their performance (www. usinessdictionary. com). Everyone need to feel empowered regarding their work ethic on the job or being assertive in life challenges and changes, but without the key elements of self-motivation, assertiveness and the ability to make good decision how would they be able to motivate others. Mangers of an organization should show through their behavior and actions how provide the necessary skills to be productive and efficient. This in turn will produce hap pier employees. Define the components of empowerment In order to be an effective leader a person would need to be empowering by those below them and therefore allow them to take initiative on their own. A leader that has poor management skill fails to empower anyone underneath him. For example, an office manager as well as a beginner officer within the work place or in the field must feel good about performing on their own and not feeling they will be overly scrutinized regarding their errors. They must not fear the aftermaths or punishments for taking individual decision making. When this happens, employees began to shun management for fear always doing something wrong. Employers should provide positive communication to their staff (National Empowerment Center). There was a research designed to examine the concept word of empowerment in this programs sponsored by multiple examiner of the criminal justice it was first anticipated to create a working definition. After research the â€Å"elements of empowerment were identified, including access to information, ability to make choices, assertiveness, and self-esteem. Empowerment has both an individual and a group dimension (Tom Roger, 2011). This research studied participants in self-help programs for people with no self-motivation and the need for encouragement. At the beginning of the study,† it was stated numerous of the individuals involved empower in their research. It’s obviously the important theory concerning the essential to defining meaning of empowerment as part of the development. Although it â€Å"recognized that empowerment had elements in common with such concepts as self-esteem and self-efficacy, these concepts did not fully capture what was considered a distinctiveness about empowerment† (Tom Roger, 2011). In an earlier â€Å"debates empowerment was† considered â€Å"complex, multidimensional concept, and that it described a process rather than an event†. It was never â€Å"believe that an individual had to display every quality on the list in order to be considered† empowered (Tom Roger, 2011). Analyze the implications of empowerment and delegation in a criminal justice organization Today the style and practices of police leadership is changing rapidly. The term shared leadership is one being used by many police chiefs all across the country. They believe that this approach to management by sharing power and influence to individuals within the organization, who are otherwise hierarchical unequal, will view their leadership as a leadership which looks at the broader aspect of giving authority to employees which will allow them to make decisions, solve problems, and be held accountable for their actions (Mussellwhite, 2007). Whenever officers are empowered they become dedicated to their jobs. Because they are given the skills, resources, authority, opportunity to be motivated and committed to their jobs, roles, or duties. Something which is refers to as being dedicated. Employees empowerment in a police department which delegate assignments, allow officers to take responsibility of their jobs results. It also allows them to make decisions about it. When officers are delegated with responsibilities, it makes them think in decision making, take control, behave, and take actions responsively, because they know that they will be held accountable for their actions. Police departments that apply employee empowerment create an environment in which their officers are empowered, productive and are happy in their jobs. Such police departments do not limit their employees to information (Heathfield, 2012). Analyze the role of trust in personnel issues. The role of trust in personnel issues involves how employees relate and respond to their employer. For example, if an employer is more concerned about getting the job done employees will pick up on that and will respond to their employer in a negative way. But when employers’ show that they truly care about their employee’s well being they will respond in a positive way. It would be in the organizations best interest for employers to take time to get to know their employees. Employers should empower their staff by allowing them to make important decisions in reference to their day to day duties. This will cause employees to respect their employers which will also lead to trust. When employees trust and respect the organization they will do the best possible work they are capable of doing. This in turn will lead to accountability on the part of the employee (Mussellwhite, 2007). When an employer gains the trust of their staff, they will know how to motivate each individual person for the best outcome of the organization. Employees understand that no organization is a perfect organization. When employees know and feel important to the organization, respect and trust will deepen. Respect and trust will take an organization to higher creativity and increased efficiency (Forbes. com, 2009). Positive communication is the cornerstone to empowerment and trust within an organization. When employers take the time to consider their staff opinions and feedback on daily operations, this can cause higher productivity and increase efficiently. Employers should share information about the organization with their staff. This provides for a clear picture of how the organization is progressing or not progressing. This also produces trust among employees because employers trust their staff (Mussellwhite, 2007). Conclusion In conclusion, this paper compared motivation and empowerment, defined the components of empowerment, analyzed the implications of empowerment and delegation in a criminal justice organization and analyzed the role of trust in personnel issues. Motivation and empowerment is about providing production and management guidelines to employees and then allows them to operate in an independent manner. Empowerment and delegation within the criminal justice system involves sharing power and influence to individuals within the organization, who are otherwise hierarchical unequal. This leadership looks at the broader aspect of giving authority to employees which will allow them to make decisions, solve problems, and be held accountable for their actions. The components of empowerment are about communication, feedback and productivity. Employees should be trained properly so that trust is gained through empowerment. A motivated and empowered employee will respect and trust the organization which will lead to accountability and responsibility. Leaders can gain great achievement when they effectively motivate and empower their staff into self development of skills and abilities. References Chris Musselwhite, 2007. Inc. Motivation=Empowerment; retrieved on November 22, 2012 from www. inc. com/resources/leadership/articles/20070801 Employees Empowerment: How to Empower employees. Retrieved on November 22, 2012 from www. humanresources. about. com/od/employeeempowerment Empowerment in the Workplace ttp://ezinearticles. com/? Empowerment-in-the-Workplace&id=6541445 Make sure your employees trust you or else. Forbes. com 2009. Retrieved from http://www. forbes. com/2009/03/23/trust-respect-employees-leadership-managing-blanchard. html. National Empowerment Center Article www. power2u. org/articles/empowerment/workingdef. html Susan Heathfield. 2012. What is Empowerment; Retrieved on November 22, 20 12 from www. businessdictionary. com/definition/empowerment. html What is Motivation; Retrieved on November 22, 2012 from www. Businessdictionary. com/definition/motivation. html

Conjugating the Verb Emmener (to Take) in French

Conjugating the Verb Emmener (to Take) in French Similar to the verb,  amener  (to take or bring),  emmener  also means to take in French. This may be a simple verb, but conjugating it to the past, present, or future tense can be a little complicated. There are a few things to watch out for, which well examine in a short French lesson. Conjugating the French Verb  Emmener When a verb ends with  -e_er  like  emmener, the spelling needs to be changed for some of the conjugations. These are called  stem-changing verbs  and in many cases, the second E changes to an accented à ¨. While this may not make much difference in the pronunciation, it certainly does when youre writing it. If you pay attention to that small detail, the rest of the conjugations are easy. The infinitive endings that are attached to the verb stem are similar to those found in regular -er  verbs, which make up the majority in the French language. If you have a few of those memorized, simply apply those endings to  emmener. To conjugate  emmener  to mean taking, will take, or took,  match the subject pronoun to the appropriate tense. For instance, I am taking is jemmà ¨ne while we will take is nous emmà ¨nerons. Practicing each of these in sample sentences will help you memorize them. Subject Present Future Imperfect j' emmà ¨ne emmà ¨nerai emmenais tu emmà ¨nes emmà ¨neras emmenais il emmà ¨ne emmà ¨nera emmenait nous emmenons emmà ¨nerons emmenions vous emmenez emmà ¨nerez emmeniez ils emmà ¨nent emmà ¨neront emmenaient The Present Participle of  Emmener For  emmener, the  present participle  is  emmenant. There is no change to the verb stem, instead we simply add the ending -ant.  Not only is this a verb, it may be used as an adjective, gerund, or noun as well. The Past Participle and Passà © Composà © The past tense can be formed using either the imperfect or the  passà © composà ©. To construct the latter, conjugate the  auxiliary verb  avoir, then attach the  past participle  emmenà ©. As an example, I took is jai emmenà © and we took is nous avons emmenà ©. More Simple  Emmener  Conjugations There are a few more common conjugations of  emmener  that you may need to know. However, those discussed above should be a priority in your studies. When the verbs action is not guaranteed, you might use the subjunctive verb mood. Similarly, the conditional verb mood is used when something else needs to occur in order for the taking to happen. In formal writing, you might also encounter the passà © simple or the imperfect subjunctive. Subject Subjunctive Conditional Passà © Simple Imperfect Subjunctive j' emmà ¨ne emmà ¨nerais emmenai emmenasse tu emmà ¨nes emmà ¨nerais emmenas emmenasses il emmà ¨ne emmà ¨nerait emmena emmenà ¢t nous emmenions emmà ¨nerions emmenà ¢mes emmenassions vous emmeniez emmà ¨neriez emmenà ¢tes emmenassiez ils emmà ¨nent emmà ¨neraient emmenà ¨rent emmenassent The imperative verb form is used for  requests  and demands. When using it, keep things short and sweet and drop the subject pronoun: use emmà ¨ne rather than tu emmà ¨ne. Imperative (tu) emmà ¨ne (nous) emmenons (vous) emmenez

How Simone de Beauvoir Inspired Second Wave Feminism

How Simone de Beauvoir Inspired Second Wave Feminism â€Å"One is not born, but rather becomes, a woman.† - Simone de Beauvoir, in The Second Sex Was Simone de Beauvoir a feminist? Her landmark book The Second Sex was one of the first inspirations to the activists of the Womens Liberation Movement, even before Betty Friedan wrote The Feminine Mystique. However, Simone de Beauvoir did not at first define herself as a feminist. Liberation Through Socialist Struggle In The Second Sex, published in 1949, Simone de Beauvoir downplayed her association with feminism as she then knew it. Like many of her associates, she believed that socialist development and class struggle were needed to solve societys problems, not a womens movement. When 1960s feminists approached her, she did not rush to enthusiastically join their cause. As the resurgence and reinvention of feminism spread during the 1960s, Simone de Beauvoir noted that socialist development had not left women better off in the USSR or in China than they were in capitalist countries. Soviet women had jobs and government positions but were still unfailingly the ones attending to the housework and children at the end of the workday. This, she recognized, mirrored the problems being discussed by feminists in the United States about housewives and womens roles. The Need for a Womens Movement In a 1972 interview with Alice Schwarzer, Simone de Beauvoir declared that she really was a feminist. She called her rejection of a womens movement a shortcoming of The Second Sex. She also said the most important thing women can do in their lives is work, so they can be independent. Work was not perfect, nor was it a solution to all problems, but it was the first condition for womens independence, according to Simone de Beauvoir. She lived in France, but Simone de Beauvoir continued to read and examine the writings of prominent U.S. feminist theorists such as Shulamith Firestone and Kate Millett. Simone de Beauvoir also theorized that women could not be truly liberated until the system of patriarchal society itself was overthrown. Yes, women needed to be liberated individually, but they also needed to fight in solidarity with the political left and the working classes. Her ideas were compatible with the belief that the personal is political. No Separate Womens Nature Later in the 1970s, Simone de Beauvoir, as a feminist, was dismayed by the idea of a separate, mystical feminine nature, a New Age concept that seemed to be gaining popularity. Just as I do not believe that women are inferior to men by nature, nor do I believe that they are their natural superiors either.- Simone de Beauvoir, in 1976 In The Second Sex, Simone de Beauvoir had famously stated, One is not born, but rather becomes, a woman. Women are different from men because of what they have been taught and socialized to do and be. It was dangerous, she said, to imagine an eternal feminine nature, in which women were more in touch with the earth and the cycles of the moon. According to Simone de Beauvoir, this was just another way for men to control women, by telling women they are better off in their cosmic, spiritual eternal feminine, kept away from mens knowledge and left without all the mens concerns like work, careers, and power. A Return to Enslavement The notion of a womans nature struck Simone de Beauvoir as further oppression. She called motherhood a way of turning women into slaves. It did not have to be that way, but it usually ended up that way in society precisely because women were told to concern themselves with their divine nature. They were forced to focus on motherhood and femininity instead of politics, technology or anything else outside of home and family. Given that one can hardly tell women that washing up saucepans is their divine mission, they are told that bringing up children is their divine mission.- Simone de Beauvoir, in 1982 This was a way of rendering women second-class citizens: the second sex. Transformation of Society The Womens Liberation Movement helped Simone de Beauvoir become more attuned to the day-to-day sexism women experienced. Yet, she did not think it was beneficial for women to refuse to do anything the mans way or refuse to take on qualities deemed masculine. Some radical feminist organizations rejected leadership hierarchy as a reflection of masculine authority and said no single person was in charge. Some feminist artists declared they could never truly create unless they were completely separate from male-dominated art. Simone de Beauvoir recognized that Womens Liberation had done some good, but she said feminists should not utterly reject being a part of the mans world, whether in organizational power or with their creative work. From Simone de Beauvoirs point of view, the work of feminism was to transform society and womens place in it. Read more of Alice Schwarzers interviews with Simone de Beauvoir in her book After the Second Sex: Conversations With Simone de Beauvoir, published by Pantheon Books in 1984.)

Questions on Investment Essay Example | Topics and Well Written Essays - 2000 words

Questions on Investment - Essay Example With the combination of these securities in the portfolio investors can minimize their risk without sacrificing their expected return. Ans 1b In table 1, we can see correlation coefficients of five different funds, if we want to invest in any of the two funds in the portfolio with minimum risk. For example Aviva Property and HSBC Asian Growth with correlation coefficient 0.10 can minimize the risk without sacrificing any of their expected return. In such a combination of these funds the risk will be diversified effectively without decreasing the level of expected return whatsoever. Ans 1c We have a portfolio of two funds, Aviva property and HSBC Asian Growth. We have also combined other funds and analyzed their risk exposure and found that their portfolio has minimum levels of risk. This investor is investing 50% of the cash in both the funds minimizing its risk without change in the expected return. Aviva property has risk of 2.3% with negative return while HSBC shows 6.5% risk with a good level of return. If investors will invests separately in these funds, the risk will be more but in other combinations the risk will also be greater. Ans 1d i) As shown in figure 1, the curved line denoted with (a) is referred to as Efficient frontier while the straight line denoted by b is referred to as capital market line. The other points mentioned in the figure are, D which is the risk free rate and C is the market portfolio. ii) When an investor has a portfolio at point E as shown in figure 1, where return is also decreasing but not that much with the risk. Every investor wants to hold the C point where only market risk is to be faced, but all the other risks are diversified and at point E the risk associated with the portfolio is high but both systematic and unsystematic risk are in its way. Only unsystematic risk is diversifiable but other risks can also be shown at beta. iii) Every investor tries to reduce the risk associated with its portfolio. If the investor is wi lling to accept 10% of risk as shown in figure 1 it is difficult to get good results. As the risk is high and return is also high, this position becomes more sensitive to the market fluctuations. At this position a slight change in the market will affect the portfolio more. Investors can go for the risk free return but it is usually unrealistic as allowances for the differences in borrowing makes the model more complicated. Ans 2a In US-style option, the buyer has the right to exercise at any time before the expiry of the option and counterparty must follow in the part of the buyer of option for execution. Counterparty doesn’t have any right to deny the execution of the option before the expiry of the risk associated with the counterparty. The reason for counterparty risk is basically due to the credit risk of the option writer (Klein and Yang 2010, p.1) Ans 2b The biggest advantage of using options contracts is that it gives stability and security to the investors and the tr aders. This makes them contribute more in the market operations and also greatly contribute to the economy. It reduces the risks involved and also increase investors confidence thus they buy more options the agents sells. Since unsystematic risk is normally unverifiable, the risk occurs due to external factors which are unpredictable. In order to minimize

International Financial Management Essay Example | Topics and Well Written Essays - 2500 words

International Financial Management - Essay Example Interest rate swaps are especially useful where on one hand, a firm wants to receive/make payment in the form of a variable interest rate and on the other hand another firm which prefers instead to receive/make payment in the form of fixed interest rate so as to limit its future risk. The first swap was executed over thirty years ago (Corb, 2012). The rationale behind such a derivative instrument is that, both parties to the financial arrangement have their own distinct priorities and requirements such that in swapping, there is a mutual benefit to be derived. This benefit arises from three major elements of the capital market: The comparative advantage, information asymmetries and fixed rate debt vis-a-vis the embedded options (Flavell, 2010). In essence, the monetary gain one party makes through the swap contract is equal to the monetary loss of the counterparty to the contract. This is to say that although there is the overall benefit of a minimized risk arising from uncertainties within the financial market, one party to the contract will incur some monetary loss. ... The most common forms of interest rate swaps include: Fixed for floating interest rate swap Floating for fixed interest swaps Same currency swaps Different currency swaps Discussion For firms such as ABC limited, a variable for fixed interest rate swap is very desirable. Firstly, with regard to synthetic fixed rate financing (also referred to as signaling). The asymmetric nature of the information environment means that firms themselves possess a better view of their levels of credit risks. As such, they require a credible way(s) of transmitting such information to the investors within the market. The firm’s borrowing of a short term debt instrument and swapping it for a fixed debt instrument signals good levels of credit of the firm to the market (Flavell, 2010). A firm is only able to do this in light of its improving future prospects. Any subsequent floating/variable debt instruments sought after will be at better and better rates (since the market can in itself recognize t his) provided that the market is sure that the firm’s projected level of credit is sound. Ordinarily, the market reacts harshly to any false signaling by firms about their credit levels. The market conducts a comparison of the firm’s signal now and its performance in the subsequent period; where the firm’s credit has not risen, the market assumes that the signaling was false and retrospectively the market may downgrade the firm’s credit rating by more than usual. Secondly, the underlying principal is not exchanged or swapped. This means that the maximum loss is substantially minimized to the net payments to the counterparties of the swap contract. Additionally, where the interest rate on floating debt

Comparing and Contrasting General Systems Theories Research Paper

Comparing and Contrasting General Systems Theories - Research Paper Example Mainly, the organizations whose their basic parts are elements of diverse orientation usually differing with the environment they are meant to operate and the relationship which exists amid them. However, in the core operation of the system it may be almost the same but as the system upgrades, to assume its core purpose it changes its modeling explanation that may have diverse mathematical modeling (Shaw, 2009). Information Systems Theory (IST) prior to the current connotation was principally limited to computer science (Von Bertalanffy, 2008). This encompasses computer organizing information, which today the term â€Å"information† is more general and requires more expounding and keen elucidation. Since, each system despite its core role is information centered; processing it for more effective operations meant to fulfill its main value as a system. IST in its actual argument, it implies that system as symbol encapsulated with an idiom. An Idiom refers to an intangible concep t mainly formulated to simply explanations for system symbols and their links. This offers a conceptual link amid set theory plus algebra especially in mathematical explanation (Von Bertalanffy, 2008). ... here it underlies all other entities to enhance more communication, hence offering effective linkage and relations of the entities and the common information space (Skyttner, 2006). These entities interacts further yielding to more multifaceted permutations. For instance, English language an idiom that is a universal concept primarily participates in offering space for the creation of sentences words and other correlated tasks, which will enable the stated language, emerge effectively, thus making understanding better. In mathematics, there a theoretical idiom that allows for the formation of mathematical statements and it accomplishment, hence proving the universal character of an idiom (Von Bertalanffy, 2008). Systems’ thinking, which yielded from Ludwig’s scholarly leaps in 1930s, meant to serve in the WWII (Skyttner, 2006). This was especially in the logistics in ensuring effective attainment of exact soldiers’ population as the war proceeded. System thinking implies how diverse entities in a certain environment co-exist without any dictation from any part entity. It involves dilemma resolution, essentially by having wide perspective of â€Å"problems† as a whole system rather than definite part, results or tasks that may prompt to additional inadvertent consequences (Skyttner, 2006). This proves that system thinking cannot be singular, but set of numerous aspects. These mainly, comprise habits or practices, which make the co-existing entities, live harmoniously. Consequently, implying that the systems parts can best understood by observing or studying their relationships and interactions they poses, which expounds unmistakably their condition of information (Von Bertalanffy, 2008). System’s components also depicts information in the manner interact with

GFP Practical Report

GFP Practical Report GFP is very useful as a reporter protein. After its discovery in 1962 its practical applications were put into use 30 years later by adding the coding DNA of GFP before the stop codon of other proteins. This allows for an easily detectable marker of the proteins presence without needing additional cofactors or causing any harm to the organism. The spectral characteristics of GFP can be changed by making mutations to the protein. In this investigation a Y66W mutation was made to wildtype GFP in order to produce a shorter excitation and emission wavelength. The mutation was made using QuikChange site directed mutagenesis. The protein was then cloned into BL21(DE3) pLysS for expression. The cells were then lysed and applied to a Ni-NTA column. This fractionated the lysate in order to analyse these fractions using SDS-PAGE, fluorescence and Bradford assays. It was found that the Y66W mutation was successfully added but due to another mutation in the stop codon additional amino acids were added to the C terminus of the protein. It was also found that purification was partially successful as GFP was eluted in the correct fraction. This is supported by the Bradford and fluorescence assays. The green fluorescent protein (GFP) is a 238 amino acid protein with a molecular mass of 26,870 Da. It was first isolated from the jellyfish species Aequorea Victoria by Osamu Shimomura in 1962 (1). GFP is expressed in small photoorgans that are situated in the umbrella of the jellyfish. Douglas Prasher first realised the potential of GFP as a reporter protein (2). As proteins are smaller than the resolving power of electron microscopes, Prasher thought the GFP gene could be added into the gene for haemoglobin before the stop codon. This would allow the protein of interest to maintain all of its functions but would have the GFP protein at its C terminal end. This means that detection of GFP fluorescence would also indicate the presence of haemoglobin. Furthermore, GFP does not require additional cofactors or substrates to fluoresce. This means that it works extremely well as a non-invasive method of detection of protein expression. GFP is also non-toxic so it is able to be used in vi vo without causing damage or harm to the organism. Crystallisation studies (3) have shown that GFP has a barrel structure with the chromophore buried in the centre. This chromophore is comprised of 3 amino acids (Ser 65-Tyr 66-Gly 67) that undergo a series of spontaneous cyclisation reactions to create the active chromophore. Wild type GFP has a major excitation peak at 395 nm and a minor one at 475 nm with an emission peak at 509 nm. In vivo GFP is coupled to the protein aequorin which induces a blue glow when it interacts with Ca2+ ions and breaks down luciferin. This light is able to excite GFP and cause fluorescence. In vitro this is not the case, however GFP fluorescence can be easily induced by irradiating GFP with UV light. As with all proteins, GFP can be mutated. By mutating key residues, such as residues in the chromophore, it is possible to change the characteristics of GFPs fluorescence. The first of many mutations was the S65T mutation (4). This mutation improved the characteristics of the protein including increased photostability, fluorescence and a shift of the major excitation peak. This investigation is based on the engineering of GFP to create a mutant of GFP with a shorter excitation and emission wavelength by inducing the Y66W mutation. The aims of this investigation were as follows. To carry out site directed mutagenesis of GFPuv to clone into pET28c and transform the products into XL-1 super competent cells. Extraction of the plasmid after incubation overnight to check the purity and concentration of DNA. Preparation and transformation of BL21(DE3) cells. Lyse these cells and fractionate the lysate to purify his tagged GFP using a Ni-NTA column. Finally, detection of purified GFP by SDS- PAGE, Bradford assay and fluorescence. The workflow of the investigation can be found in figure 1 in appendix 1. A more detailed protocol can be found in the BIOC2302 semester 2 practical manual on pages 6-15 with rationale for all experiments. In site directed mutagenesis I 31 ÃŽ ¼l of water was added to the PCR reaction to give a total reaction volume of 50 ÃŽ ¼l. In site directed mutagenesis II a supplied culture of cells was used in the experiment rather than cells from the transformation colonies in site directed mutagenesis I. His tagged GFP was used instead of the mutant in the protein purification experiment in order for easier administration as the process is the same. Site directed mutagenesis I Before the wet lab work began it was first necessary to design primers for QuikChange to induce the Y66W mutation into the wild type GFP. These can be seen as figure 2 in appendix 2. These were created using the QuikChange primer design tool on the Agilent website. The site directed mutagenesis was carried out using the primers supplied to induce the correct mutation. The products of this were cloned into the pET28c plasmid and the XL-1 super competent cells. The cells were plated as per the BIOC2302 practical manual and left to incubate overnight. Site directed mutagenesis II Upon checking the plates in the next session it was found that no transformed colonies had grown so a new culture was supplied. The undigested plasmid control grew approximately 50 colonies The culture of BL21(DE3)pLysS cells was set up and the OD600 were recorded. They can be seen in table 1 in appendix 3. Within 50 minutes the culture had reached an OD600 of 0.483 meaning the cells were at the correct density for lysis. The cells were prepared as per the BIOC2302 practical manual and the recombinant plasmid was extracted. The concentration measured was 121.7 ng/ÃŽ ¼l and the A260/A280 was 1.86 using nanodrop. Therefore, the ethanol precipitation was not carried out. To prepare for sequencing 4.11 ÃŽ ¼l of this solution was diluted, with 5.98 ÃŽ ¼l EB buffer, to the correct concentration. This was then sent to be sequenced, the results of which can be seen in appendix 4. The primer has been highlighted in green and is surrounded by a box with the mutated codon in red. A deletion also occurred in the stop codon of the mutant as highlighted by the second box with deleted bases highlighted in blue. Protein purification The plates were inspected in the next session. It was found that the 200 ÃŽ ¼l transformation plate grew 3 colonies and the 50 ÃŽ ¼l transformation plate grew none. Transformation efficiency can be calculated for the 200 ÃŽ ¼l plate as 37 transformants/ÃŽ ¼g of DNA. The cells were weighed and found to be 0.539 g so 2 ml BugbusterTM used. After lysis and fractionation the SDS-PAGE samples of each fraction were prepared and loaded onto the gel. The Bradford assay was carried out while the gel ran.   The BSA standards were calculated and the contents of each standard well can be seen in table 2 in appendix 3. The fractions were then diluted into their wells and the contents can be seen in table 3 in appendix 3. The plate was filled according to the map in figure 2 in appendix 5. The plate was ran and the absorbances for the BSA standards were taken from the plate readout and inputted into table 4 in appendix 5. From here a calibration graph was set up using GraphPad Prism and can be seen as graph 1 in appendix 6. This graph shows that the data points for the standards do not fall near the line of best fit. The absorbance results from the plate readout for all of the fractions were imputed into table 5 in appendix 7. The equation of the line from graph 1 was then used to calculate the concentration of protein in each of the fractions. All of these values were also inputted into table 5. With the Bradford assay complete the SDS-PAGE gel was disassembled, stained and a picture was taken. A map of the gel can be seen as figure 3 in appendix 7 and the picture of the gel can be seen as figure 4 in appendix 8. By looking at the picture it can be seen that in lanes 2, 3, 4 and 9 there are dark bands spanning the entire lane. In 5, 6 and 8 there is faint banding across the well. In well 7 there is a distinct small band in between the 25 kDa and 37 kDa molecular markers. Lane 8 shows no bands at all. Finally the fluorescence assay was carried out as per the map of the microtiter plate in figure 5 in appendix 7. The results from the plate readout were inputted into table 5. From here a graph comparing the log of protein concentration compared to fluorescence of each fraction was plotted and can be found as graph 2 in appendix 6. This shows elution 1 with the highest fluorescence and the unbound x10 had the lowest. However, when comparing protein concentration the unbound fraction had the highest and wash 2 had none. Percentage fluorescence was also calculated and inputted into table 6 in appendix 9. The first aim of this experiment was to transform the site directed mutagenesis products into XL-1 super competent cells. The correct primers were used in order to induce the Y66W mutation into the parental DNA. However, no colonies that were meant to take up the mutated plasmid grew but the undigested control grew around 50 colonies. This means the cells did not take up the plasmid because otherwise they would have grown on the plate. This could be due to a mistake made in making the PCR reaction mixture or the DNA may have become damaged at some point in the experiment. Additionally, the suppliers of the XL-1 super competent cells advice to avoid large changes in temperature. This was unavoidable in this experiment and may have contributed to the cells not taking up the plasmid. In the future more care should be taken while plating and preparing the cells. Also preparation of any reaction mixtures should be checked very closely in order to ensure the correct reactants are added in the correct amounts. In site directed mutagenesis II the cell culture was lysed when the OD600 was 0.483. That is because E.coli cells are most likely to be made competent when they enter early log phase. This corresponds with an OD600 of 0.4-0.5. The DNA concentration extracted in this experiment was found to be 121.7 ng/ÃŽ ¼l and an A260/A280 of 1.86. This means that the DNA is good quality as the desirable range for A260/A280 is 1.7-2.0 and the concentration was much higher that what was required. However, in future experiments to test for reliability multiple results should be taken. Furthermore, the data could have been confirmed by using the spectrophotometric method alongside using nanodrop. The sequencing results in appendix 4 confirmed the successful incorporation of the Y66W mutation into GFP, creating the CFP mutant. However, the second mutation at the stop codon deleted 2 bases including the first base of the stop codon. This means that when the protein is expressed the ribosome will not stop and instead will continue to add amino acids onto the C terminus of the mutant until it reaches a new stop codon. There 144 bases between the original stop codon and the next in frame stop codon meaning 48 additional amino acids will be added to the C terminus. This codon can be seen highlighted in purple below the original stop codon. These additional amino acids could affect the folding or could increase the likelihood of aggregation of the mutant protein. In the protein purification experiment the 200 ÃŽ ¼l transformation plate grew 3 colonies and the 50 ÃŽ ¼l transformation plate grew none. The transformation efficiency on the 200 ÃŽ ¼l plate was 37 transformants/ÃŽ ¼g of DNA. The reason why this is so low could be due to a number of factors such as the plating technique or the cells may not have been left to chill on ice for the optimum amount of time. However, the negative control did not grow any colonies, confirming that all of the bacteria on the transformation plate were transformed. Again, more steps should be taken if this was to be carried out again to ensure that proper plating and prepping protocol is followed. The Bradford assay shows that in wash 2 there was no protein in the well. This means that any protein found in elutions 1 and 2 should all be His tagged GFP that bound the Ni-NTA column. This can be confirmed by the fluorescence results as the elution 1 fraction contained the majority of the total fluorescence with 44.13% of the total. However, all other fractions also produce some fluorescence. This could be due to GFP contamination in the other fractions. This could have occurred due to the resin being saturated, preventing further binding to the column. It could also be due to aggregation of the protein obscuring the His tag and preventing binding. Furthermore, the plots on the calibration graph do not fall on the line of best fit. This means that the equation of the line is not accurate and protein concentrations calculated using it are also inaccurate. Therefore, there could be more protein in each fraction than was calculated. This could account for the fluorescence in the wash and unbound fractions. The Bradford assay is quite limiting. This is due to the fact the assay only measures protein concentration rather than GFP concentration. This means that it is unsure whether the protein concentration measured in elution 1 and 2 is all GFP or it is contaminant protein. The same can be said for the other fractions, its unsure whether the protein concentration measured has been contaminated by GFP. In the future this assay should be carried out again to try and reduce contamination. The calibration graph should also be repeated until all of the data points fall on the line of best fit. Otherwise none of the calculated protein concentrations are accurate. Finally, the SDS-PAGE results shows banding in wash 1, 2 and elution 1 and 2. This suggests that there is contaminating protein in all of these fractions. Elution 1 shows a clear band at approximately the 26-27 kDa mark as it is present just above the 25 kDa marker and is well below the 37 kDa marker. This suggest the band in elution 1 is GFP as it is the appropriate size and is in the expected fraction. Another source of error could be due to the amount of pressure applied to the pipette. This will vary from person to person and will affect the volume of the solution being pipetted. As such small volumes were being used and there was a lot of solutions to be pipetted it is very possible a mistake was made. This mistake would have a big effect on the concentration and therefore could have a big effect on the absorbance values. These errors can be avoided in future by using the appropriate pipette for the volumes being used. Further reduction in errors can come from correct technique and by doing replicates and averaging values. There could be some error in the microtiter itself. There may have been markings or scratches on the plate that werent seen at the time. This could affect how the light passed through the reader and therefore affect the absorbance values. In conclusion, the aims of this investigation were to induce the Y66W mutation into wild type GFP using QuikChange site directed mutagenesis. Furthermore, the protein was to be expressed in competent BL21(DE3) pLysS cells. Finally wildtype GFP was to be purified using a Ni-NTA column and the fractions analysed with SDS-PAGE, fluorescence and Bradford assays. The investigation successfully introduced the Y66W mutation into wildtype GFP. However, the stop codon was also mutated adding an extra 48 amino acids on the C terminal of the protein.   A band indicating the presence of GFP was found at the 26.9 kDa mark in elution 1, indicating it was bound to the column and was eluted. However, all factions were contaminated with other protein. References      Ã‚   1. Shimomura, O., Johnson, F., and Saiga, Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous. s.l. : J. Cell. Comp. Physiol. 59:223-39, 1962. 2. Prasher, D., Eckenrode, V., Ward, W., Prendergast, F., and Cormier, M. Primary structure of the Aequorea Victoria green-fluorescent protein. s.l. : Gene 111 (2)229-33, 1992. 3. Ormo, M., Cubitt, A., Kallio, K., Gross, L., Tsien, R., and Remington. S. Crystal structure of the Aequorea Victoria green fluorescent protein. s.l. : Science 273:1392-5, 1996. 4. Heim R, Cubitt AB, Tsien RY. Improved green fluroescence. s.l. : Nature. 373 (6516): 663-4., 1995.